Journal: International Journal of Molecular Sciences
Article Title: Efficacy of Cannabidiol in Reducing Virulence of Listeria monocytogenes
doi: 10.3390/ijms27062682
Figure Lengend Snippet: ( a ) Effect of cannabidiol on the adhesion of L. monocytogenes Scott A onto Caco-2 cells. ( b ) Effect of cannabidiol on the adhesion of L. monocytogenes ATCC 19115 onto Caco-2 cells. L. monocytogenes cultures (~7 log CFU/mL) pre-exposed to CBD for 5 h were used to infect Caco-2 monolayers (Multiplicity of Infection (MOI) of 1:100) for 1 h, followed by washing, cell lysis, and enumeration of adherent bacteria by plating. Bars represent the mean adhesion on Caco-2 cells (%) ± SD (n = 6). Different superscript letters indicate significant differences across treatments compared to the control ( p < 0.05). Abbreviations: DMSO, dimethyl sulfoxide (solvent control); SIC, sub-inhibitory concentration; MIC, minimum inhibitory concentration.
Article Snippet: The effect of CBD on LM adhesion and invasion of host cells was determined using two different cell lines, namely human enterocyte-like Caco-2 cells (ATCC HTB-27) and human brain microvascular endothelial cells (HBMEC).
Techniques: Infection, Lysis, Bacteria, Control, Solvent, Concentration Assay